Products for Translation in Cells


mRNA Production


T7 mScript™ Standard mRNA Production System 
(PDF)
Simply the best way to make in vitro mRNA. Combines the benefits of high-yield transcription with those of post-transcriptional capping and tailing all in one kit. See T7-FlashScribe Transcription Kit, ScriptCap m7G Capping System, ScriptCap 2'-O-Methyltransferase Kit and A-Plus Poly(A) Polymerase Tailing Kit on this page.
C-MSC11610 10 reactions $320
C-MSC100625 25 reactions $702


In Vitro Transcription


T7-FlashScribe™ Transcription Kit 
(PDF)
For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in
30 minutes.
C-ASF3507 50 reactions $286

T7-Scribe™ Standard RNA IVT Kit 
(PDF)
For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours.
C-AS3107 50 reactions $267

SP6-Scribe™ Standard RNA IVT Kit 
(PDF)
For the production of in vitro transcribed RNA with SP6 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 90 µg RNA from 1 µg DNA in 2 hours.
C-AS3106 50 reactions $267


Post-Transcriptional Capping


ScriptCap™ m7G Capping System 
(PDF)
For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produce
Cap 1 caps, that which is found on most eukaryotic mRNAs.
C-SCCE0625 25 reactions $436

ScriptCap™ 2'-O-Methyltransferase Kit 
(PDF)
For conversion of RNA containing Cap 0 caps to those containing Cap 1 caps, which are found on most eukaryotic mRNAs. Can be used simultaneously with ScriptCap m7G Capping System to produce virtually 100% Cap 1-capped RNA in a single reaction.
C-SCMT0625 25 reactions $219

ScriptCap™ Cap 1 Capping System 
(PDF)
For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2'-O-Methyltransferase, GTP and SAM. Either Cap 0 or Cap 1 caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods.
C-SCCS1710 10 reactions $211


Co-Transcriptional Capping


MessageMAX™ T7 ARCA-Capped Message Transcription Kit 
(PDF)
For co-transcriptional capping of IVT RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). A reaction produces 60 µg RNA from 1 µg DNA in 30 minutes. Reaction products are 80% correctly capped and 20% uncapped.
C-MMA60710 10 reactions $323

AmpliCap-Max™ T7 High Yield Message Maker Kit 
(PDF)
For co-transcriptional capping of IVT RNA with T7 RNA polymerase and standard cap analog. A reaction produces 60 µg RNA from 1 µg DNA in 30 minutes. Reaction products are 40% correctly capped, 40% incorrectly capped and 20% uncapped.
C-ACM04037 25 reactions $368

AmpliCap™ SP6 High Yield Message Maker Kit 
(PDF)
For co-transcriptional capping of IVT RNA with SP6 RNA polymerase and standard cap analog. A reaction produces 35 µg RNA from 1 µg DNA in 2 hours. Reaction products are 40% correctly capped, 40% incorrectly capped and 20% uncapped.
C-AC0706 25 reactions $368


Post-Transcriptional Tailing


A-Plus™ Poly(A) Polymerase Tailing Kit 
(PDF)
For post-transcriptional poly(A) tail addition to RNAs. Allows tailing of mRNAs
in vitro, to lengths equivalent to or greater than those found in nature. This cannot be accomplished with co-transcriptional tailing methods.
C-PAP5104H 50 reactions $219


Modified NTP In Vitro Transcription


INCOGNITO™ T7 Ψ-RNA Transcription Kit 
(PDF)
For the production of pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours.
C-ICTY110510 10 reactions $410

INCOGNITO™ SP6 Ψ-RNA Transcription Kit 
(PDF)
For the production of pseudouridine-containing, in vitro transcribed RNA with SP6 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 60 µg RNA from 1 µg DNA in 2 hours.
C-ICSY110510 10 reactions $410

INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit 
(PDF)
For the production of 5-methylcytosine- pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours.
C-ICTMY110510 10 reactions $691

INCOGNITO™ T7 ARCA 5mC- & Ψ-RNA Transcription Kit 
(PDF)
For co-transcriptional capping of 5-methylcytosine- pseudouridine-containing,
in vitro transcribed RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 40 µg RNA from 1 µg DNA in 3 hours. Reaction products are 80% correctly capped and 20% uncapped.
C-ICTAMY110510 10 reactions $842


mRNA Decapping


Cap-Clip™ Acid Pyrophosphatase 
(PDF)
For the removal of cap structures (decapping) from various RNAs (i.e. mRNAs) resulting in 5'-monophosphate RNAs. Can be substituted unit for unit, into any preexisting protocol for Tobacco Acid Pyrophosphatase (TAP) utilizing the same buffer.
C-CC15011H 100 units $550


RNase Inhibition


ScriptGuard™ RNase Inhibitor 
(PDF)
Inhibits eukaryotic RNases A, B and C. Use as a safeguard against RNA degradation in any RNA-containing reaction.
C-SRI6310K 10,000 units $278

The Products listed above are currently offered by CELLSCRIPT™ for research use only under the defined Terms and Conditions. To place an order, visit How To Order.

Contact CELLSCRIPT™ to inquire about customizations of existing products or manufacturing of products for other uses, such as therapeutics, which may require meeting regulatory standards, such as use of components that are not derived from animal origin.